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ELISA Kit for Complement Factor I (CFI)

CAT: 0338-TRV-049509-03Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-049509-03Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Product Name Alternative
CF-I; C3b-INA; FI; IF; KAF; Factor I; Konglutinogen-Activating Factor; C3b-Inactivator; C3B/C4B inactivator
UniProt
Q9WUW3
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Metabolic pathway; Immune molecule; Hematology; Autoimmunity
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor I (CFI) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor I (CFI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor I (CFI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor I (CFI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Assay Performance Time
3h
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Factor I (CFI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Factor I (CFI) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 0.239ng/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Factor I (CFI) . No significant cross-reactivity or interference between Complement Factor I (CFI) and analogues was observed.
Product Name Synonym
Complement Factor I
Method
Double-antibody Sandwich
Organism Species
Rattus norvegicus (Rat)

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

UniProtKB · Q9WUW3

Complement factor I

CFAI_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q9WUW3
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cfi
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
3.4.21.45
Processing
Precursor
Secondary accessions
Q5EBC4
Protein keywords

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Biological process

Complement pathwayImmunityInnate immunity

Molecular function

HydrolaseProteaseSerine protease

Technical term

Reference proteome

Domain

RepeatSignal

Cellular component

Secreted

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