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ELISA Kit for Sortilin 1 (SORT1)

CAT: 0338-TRV-048080-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-048080-01Size:24 Tests
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CAS Number
7732-18-5
Product Name Alternative
NT3; Gp95; NTR3; 100 kDa NT receptor; Glycoprotein 95; Neurotensin receptor 3
UniProt
Q6PHU5
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Field of Research
Neuro science
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sortilin 1 (SORT1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sortilin 1 (SORT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sortilin 1 (SORT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sortilin 1 (SORT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
Assay Performance Time
3h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Sortilin 1 (SORT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Sortilin 1 (SORT1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 0.061ng/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Sortilin 1 (SORT1) . No significant cross-reactivity or interference between Sortilin 1 (SORT1) and analogues was observed.
Product Name Synonym
Sortilin 1
Method
Double-antibody Sandwich
Organism Species
Mus musculus (Mouse)

UniProtKB · Q6PHU5

Sortilin

SORT_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q6PHU5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Sort1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A2AEE8, Q3UHE2, Q8K043, Q9QXW6
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneEndoplasmic reticulumEndosomeGolgi apparatusLysosomeMembraneNucleus

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Molecular function

Developmental proteinReceptor

Biological process

DifferentiationEndocytosisOsteogenesisProtein transportTransport

Domain

RepeatSignalTransmembraneTransmembrane helix

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