CLIA Kit for Complement Component 8g (C8g)

CAT: 0338-TRV-051413-03Size: 96 TestsDry Ice: NoHazardous: No

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CAT#:0338-TRV-051413-03Size:96 Tests

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Applications

Chemiluminescent immunoassay for Antigen Detection.

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 8g (C8g) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 8g (C8g) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 8g (C8g) level in the sample or standard.;

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.

Assay Performance Time

2H, 40min

Sample Type

Serum, plasma and other biological fluids

Detection Range

6.86 - 5000 pg/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Component 8g (C8g) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Component 8g (C8g) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Sensitivity

The minimum detectable dose of this kit is typically less than 2.69pg/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Specificity

This assay has high sensitivity and excellent specificity for detection of Complement Component 8g (C8g) .
No significant cross-reactivity or interference between Complement Component 8g (C8g) and analogues was observed.

Method

Double-Antibody Sandwich

Organism Species

Homo sapiens (Human)

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CLIA Kit for Complement Component 8g (C8g)

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