RegisterLogin

CLIA Kit for Complement Component 3a (C3a)

CAT: 0338-GTR04126901-01Size: 24 TestsDry Ice: NoHazardous: No
CAT#:0338-GTR04126901-01Size:24 Tests
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
UniProt
P01024
Applications
Chemiluminescent immunoassay for Antigen Detection.
Field of Research
Infection immunity; Immune molecule; Autoimmunity
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 3a (C3a) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 3a (C3a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 3a (C3a) level in the sample or standard.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Assay Performance Time
2h, 40min
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
27.4-20,000pg/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Complement Component 3a (C3a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Complement Component 3a (C3a) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 11.5pg/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Component 3a (C3a) . No significant cross-reactivity or interference between Complement Component 3a (C3a) and analogues was observed.
Product Name Synonym
Complement Component 3a
Method
Double-antibody Sandwich
Organism Species
Homo sapiens (Human)

Related Products

CatalogName
GTR04488473-01ELISA Kit for Complement Component 3a (C3a)
GTR04549640-01ELISA Kit for Complement Component 3a (C3a)
GTR04380983-01ELISA Kit for Complement Component 3a (C3a)
GTR04263951-01ELISA Kit for Complement Component 3a (C3a)
GTR04539300-01ELISA Kit for Complement Component 3a (C3a)
GTR04146460-01ELISA Kit for Complement Component 3a (C3a)
GTR04479704-01Recombinant Complement Component 3a (C3a)
GTR04423675-01Recombinant Complement Component 3a (C3a)
GTR15752643-01Eukaryotic Complement Component 3a (C3a)
GTR04316699-01Recombinant Complement Component 3a (C3a)

Alternative Products

CatalogName
MBS2161992-01Rat Complement Component 3a (C3a) CLIA Kit
abx491425-01Mouse Complement Component 3a (C3a) CLIA Kit
abx491426-01Pig Complement Component 3a (C3a) CLIA Kit
MBS2708752-01Human Complement Component 3a (C3a) CLIA Kit
abx491424-01Dog Complement Component 3a (C3a) CLIA Kit
abx491428-01Rabbit Complement Component 3a (C3a) CLIA Kit
AP76294-01Recombinant Mouse Complement Component C3a/C3a
32-8785-50Recombinant Human Complement Component C3a/C3a
P514448Complement Component C3a; C3a Protein (Mouse)
151684Complement Component 3a (C3a)

Popular Products