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CLIA Kit for Hemoglobin (HB)

CAT: 0338-TRV-048800-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-048800-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Hgb; Haemoglobin; Heterotetramer (αβ) 2
Applications
Chemiluminescent immunoassay for Antigen Detection.
Field of Research
Metabolic pathway; Infection immunity; Hematology; Neuro science
Assay Principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Hemoglobin (HB) . A competitive inhibition reaction is launched between biotin labeled Hemoglobin (HB) and unlabeled Hemoglobin (HB) (Standards or samples) with the pre-coated antibody specific to Hemoglobin (HB) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin (HB) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Hemoglobin (HB) level in the sample or standard.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
7. Read RLU value immediately.
Assay Performance Time
2h
Sample Type
Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids
Detection Range
1.17-300ug/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Hemoglobin (HB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Hemoglobin (HB) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 0.42ug/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Hemoglobin (HB) . No significant cross-reactivity or interference between Hemoglobin (HB) and analogues was observed.
Product Name Synonym
Hemoglobin
Method
Competitive Inhibition
Organism Species
Homo sapiens (Human)

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