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CLIA Kit for Prostate Specific Antigen (PSA)

CAT: 0338-TRV-050316-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0338-TRV-050316-01Size:24 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Product Name Alternative
KLK3; APS; KLK2A1; HK3; KLKB1; Seminin; Prostate Specific Antigen; Kallikrein-Related Peptidase 3; Semenogelase, g-Seminoprotein; P-30 Antigen; Plasma kallikrein
UniProt
P07288
Applications
Chemiluminescent immunoassay for Antigen Detection.
Field of Research
Tumor immunity; Reproductive science
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Prostate Specific Antigen (PSA) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Prostate Specific Antigen (PSA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Prostate Specific Antigen (PSA) level in the sample or standard.
Assay Protocol
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Assay Performance Time
2h, 40min
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
54.9-40,000pg/mL
Precision
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Prostate Specific Antigen (PSA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Prostate Specific Antigen (PSA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV
Sensitivity
The minimum detectable dose of this kit is typically less than 19.1pg/mL
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Format
48T, 96T, 96Tx5, 96Tx10, 96Tx100
Specificity
This assay has high sensitivity and excellent specificity for detection of Prostate Specific Antigen (PSA) . No significant cross-reactivity or interference between Prostate Specific Antigen (PSA) and analogues was observed.
Product Name Synonym
Prostate Specific Antigen
Method
Double-antibody Sandwich
Organism Species
Homo sapiens (Human)

UniProtKB · P07288

Prostate-specific antigen

KLK3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P07288
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KLK3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PSA
EC number
3.4.21.77
Processing
Precursor
Secondary accessions
C9JXH3, G3V0H4, G3XAE3, Q15096, Q16272, Q86TG8, Q8IXI4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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