C3a Mouse (TECO®)

• Description:

  C3 is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue‐specific and is modulated in response to a variety of stimulatory agents. During complement activation, C3 is proteolytically cleaved resulting in release of the anaphylatoxic peptide C3a.   C3a is a small polypeptide consisting of 74 amino acids. C3a itself is very short‐lived and in serum cleaved rapidly into the more stable C3a‐desArg (also called acylation stimulating protein, ASP) . Therefore, measurement of C3a‐desArg allows reliable conclusions about the level of complement activation in the samples.

• Assay Type:

  ELISA

• Sample Volume:

  25 μL (plasma or serum, dilute 1:100)

• Detection Range:

  31 – 500 ng/mL

• Sensitivity:

  LOD: 4.5 ng/mL LLOQ: 0.023 ng/mL

• Species:

  Mouse

• Incubation Time:

  3 Hours