Recombinant Mouse CD20/MS4A1 protein (His tag)

• Background:

  CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

• Abbreviation:

  CD20; MS4A1

• Synonyms:

  B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; CD20; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; Ms4a1

• UniProt:

  P19437

• Accession Number:

  P19437

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Leu 133-Pro 291

• Field of Research:

  Cancer; Immunolegy; Neuroscience; Stem Cells; Epigenetics and Nuclear signaling.

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  17.4 kDa

• Observed Molecular Weight:

  28 kDa

• Species:

  Mouse