Recombinant Mouse ST14/Matriptase protein (His tag)

• Background:

  Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site.Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH) . ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair.

• Abbreviation:

  ST14; Matriptase

• Synonyms:

  Epithin; HAI; MTSP1; Membrane type serine protease 1; PRSS14; Prostamin; SNC19; ST14; Serine protease 14; TADG15; TMPRSS14

• UniProt:

  P56677

• Accession Number:

  P56677

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Thr 591-Val 855

• Field of Research:

  Signal Transduction; Cancer; Cell Biology

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  29.0 kDa

• Observed Molecular Weight:

  24 kDa

• Species:

  Mouse