Recombinant Mouse S100a8 protein (His Tag)

• Background:

  S100A8 is a calcium-and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra-and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration.

• Abbreviation:

  S100a8

• Synonyms:

  60B8AG; CAGA; CFAG; CGLA; CP 10; Calgranulin A; Calprotectin L1L subunit; Cystic fibrosis antigen; L1Ag; MA387; MIF; MRP-8; MRP8; NIF; P8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium-binding protein A8; S100A8; Urinary stone protein band A

• UniProt:

  P27005

• Accession Number:

  P27005

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Pro2-Glu89

• Applications:

  Immunogen

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  11.5 kDa

• Observed Molecular Weight:

  10-11 kDa

• Species:

  Mouse