Recombinant Mouse DNMT1 Protein (His Tag)

• Background:

  Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) . Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.

• Abbreviation:

  DNMT1

• Synonyms:

  DNA (cytosine-5) -methyltransferase 1; DNA MTase MmuI; DNA methyltransferase MmuI; Dnmt; Dnmt1; M.MmuI; MCMT; Met-1; Met1; Uim

• UniProt:

  P13864

• Accession Number:

  P13864-1

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Leu1396-Pro1583

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 80% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  21.5 kDa

• Observed Molecular Weight:

  23.76 kDa

• Species:

  Mouse