Recombinant Human HIF1A Protein

• Background:

  Functions as a master transcriptional regulator of the adaptive response to hypoxia.Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.Heterodimerizes with ARNT, heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters.

• Abbreviation:

  HIF1A

• Synonyms:

  ARNT-interacting protein; BHLHE78; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; HIF-1-alpha; HIF1-alpha; Hypoxia-inducible factor 1-alpha; MOP1; Member of PAS protein 1; PAS domain-containing protein 8; PASD8; bHLHe78

• UniProt:

  Q16665

• Accession Number:

  Q16665

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Ser 576-Gln785

• Field of Research:

  Immunology; Cardiovascular

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  25.2 kDa

• Observed Molecular Weight:

  26 kDa

• Species:

  Human