Recombinant Rat IL-1α/IL-1F1 Protein (Trx Tag)

• Background:

  IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T-and B-cells) immune systems. Cytokines can be classified into two groups: pro-and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6, and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. anti-inflammatory cytokines, including IL-10, IL-4, IL-13, and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.

• Abbreviation:

  IL-1α/IL-1F1

• Synonyms:

  Interleukin; Hematopoietin; IL1F1hematopoietin; IL1F; IL-1F; alpha; BAF; Hematopoietin-1; IL1; IL1 alpha; IL-1 alpha; IL1-A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha; Il1a; FAF; Hematopoietin 1; IL 1 alpha; IL 1A; IL1; IL1 ALPHA; IL1F1; Interleukin 1 alpha; LAF; LEM; Preinterleukin 1 alpha; Il1a

• UniProt:

  P16598

• Accession Number:

  P16598

• Expression System:

  E.coli

• Tag:

  N-Trx

• Sequence:

  Ser115-Ser270

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 90% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  37 kDa

• Observed Molecular Weight:

  35 kDa

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Rat