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Fish Estrogen ELISA Kit

CAT: 0855-ELK11828-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11828-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
E ; Estrogen
Reactivity
Fish
Field of Research
Reproductive science
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fish Estrogen. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fish Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fish Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.625-1000 pg/mL
Sensitivity
4.15 pg/mL

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