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Rat C3d (Complement Fragment 3d) ELISA Kit

CAT: 0855-ELK11812-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11812-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
C3d
Reactivity
Rat
Field of Research
Immunology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C3d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C3d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C3d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C3d in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.49 ng/mL
Uniprot Accession Number
P01026

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