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Recombinant Human CD4 Protein, C-His

CAT: 1676-PR00776Size: 10μg/50μg/500μg/1mgDry Ice: NoHazardous: No
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CAT#:1676-PR00776Size:10μg/50μg/500μg/1mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CD4 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature human CD4 consists of a 371 amino acid extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. CD4 binds directly to MHC class II molecules on antigen presenting cells (10) . This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1.
Product Name Alternative
T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; sCD4
Accession Number
P01730
Tag
C-6His
Source
HEK293 Cells
Endotoxin
<1 EU/μg as determined by LAL test.
Purity
>95% as determined by reducing SDS-PAGE.
Form
Lyophilized
Reconstitution
Reconstitute the lyophilized protein in sterile water at a concentration of greater than 100 μg/mL.
Molecular Weight
41.7 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. Please minimize freeze-thaw cycles and use a manual defrost freezer.
Protein Length
Lys26-Trp390
Formulation
Lyophilized from a 0.2 μm filtered solution of 20mM Citrate,50mM NaCl,4%Trehalose,5%Mannitol,0.02% Tween80, pH6.0.
Species
Human

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