Human Interleukin-2 Recombinant

• Background:

  IL-2 is produced mainly by T- cells expressing the surface antigen CD4 following cell activation by mitogens or allogen. Interleukin-2 (IL-2) has multiple, sometimes opposing, functions during an inflammatory response. It is a potent inducer of T-cell proliferation and T- helper 1 (Th1) and Th2 effector T-cell differentiation and provides T cells with a long-lasting competitive advantage resulting in the optimal survival and function of memory cells. In a regulatory role, IL-2 is important for the development, survival, and function of regulatory T cells, it enhances Fas-mediated activation-induced cell death, and it inhibits the development of inflammatory Th17 cells. Thus, in its dual and contrasting functions, IL-2 contributes to both the induction and the termination of inflammatory immune responses.

• Description:

  Recombinant Interleukin-2 is a disulfide-linked monomer protein  encompassing amino acids 21-153, migrates as an approximately 15 kDa protein + glycanss under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-2 mature chain was expressed in CHO cells.

• UniProt:

  P60568

• Endotoxin:

  < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 95% by SDS-PAGE

• Format:

  Lyophilized protein from 0.2 μm filtered PBS solution, pH7.2,5% Trehalose.

• Reconstitution:

  Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

• Shipping Conditions:

  Gel Packs or Dry Ice

• Storage Conditions:

  The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to 3 weeks, or at -20°C for up to six months, in the presence of a carrier protein at 0.1%. Avoid repeated freeze/thaw cycles.

• Calculated Molecular Weight:

  15 kDa + glycans

• Formulation:

  Lyophilized from 0.2 μm filtered PBS solution, pH7.2,5% Trehalose.