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Rbx1/Cul4B/Dcaf11/Ddb1 Complex Recombinant

CAT: 0421-101495-1Size: 10 µgDry Ice: YesHazardous: No
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CAT#:0421-101495-1Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Eukaryotic cells have evolved complex mechanisms to regulate cellular pathways and protein quality. Cullin proteins are a family of hybrophobic proteins that serve as scaffold and can complex with RING and adaptor proteins to form CRLs (E3 ligase-Cullin-RING ligase) complexes, which can interact with different substrates and regulate multiple cellular processes. When the CRL Rbx1-Cul4b-Ddb1 binds to Dcaf11 it can target p21 for ubiquitination and regulate cell cycle progression.
Description
Complex of Rbx1 (Ring-Box 1), Cul4B (cullin 4B), Dcaf11 (Ddb1- and Cul4-associated factor 11) and Ddb1 (Damage specific DNA binding protein 1. Recombinant Rbx1, encompassing amino acids 2-108 (end), mutated at M5I, contains an N-terminal His-tag (6xHis) . Recombinant Cul4B, encompassing amino acids 3-913 (end), contains an N-terminal His-tag (6xHis) . Recombinant Dcaf11, encompassing amino acids 2-546 (end), contains an N-terminal FLAG-tag. Recombinant human Ddb1, encompassing amino acids 2-1140 (end) contains an N-terminal FLAG-tag. These recombinant proteins were co-expressed, and affinity purified.
UniProt
Rbx1: P62877, Cul4B: Q13620, Dcaf11: Q8TEB1, Ddb1: Q16531
Purity
≥ 85%
Format
Aqueous buffer solution.
Shipping Conditions
-80°C
Storage Conditions
At least 6 months at -80°C.
Calculated Molecular Weight
Rbx1: 13 kDa; Cul4B: 105 kDa; Dcaf11: 63 kDa; Ddb1: 128 kDa
Formulation
40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol

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