Phospho PKC θ (Y90) rabbit pAb

Phospho PKC θ (Y90) rabbit pAb

• Description: Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Domain: The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. enzyme regulation: Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation. function: PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. function: This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PTM: Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. similarity: Contains 1 AGC-kinase C-terminal domain. similarity: Contains 1 C2 domain. similarity: Contains 1 protein kinase domain. similarity: Contains 2 phorbol-ester/DAG-type zinc fingers. subunit: Interacts with TXNL2/PICOT. tissue specificity: Skeletal muscle, megakaryoblastic cells and platelets.
• Synonyms: Protein kinase C theta type (EC 2.7.11.13;nPKC-theta)
• Gene ID: 5588
• UniProt: Q04759
• Cellular Locus: Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthesized peptide derived from human Phospho PKC θ (Y90)
• Clonality: Polyclonal
• Validated Applications: WB, IHC
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:500-2000;IHC-p 1:50-300
• Molecular Weight: 78kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.