Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 3 (EBNA3) , partial

Recombinant Epstein-Barr virus Epstein-Barr nuclear antigen 3 (EBNA3) , partial

• CAS Number: 9000-83-3
• Gene Name: EBNA3
• UniProt: Q3KST2
• Expression Region: 1-138aa
• Organism: Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4)
• Target Sequence: MDKDRPGDPALDDNMEEEVPSTSVVQEQVSAGDWENVLIELSDSSSEKEAEDAQLEPAQKGTKRKRVDHDAGGSAPARPMLPPQPDLPGREAILRRFPLDLRTLLQAIGAAATRIDTRAIDQFFGSQISNTEMYIMYA
• Tag: N-terminal 10xHis-tagged and C-terminal Myc-tagged
• Source: E.coli
• Field of Research: Others
• Assay Type: In Stock Protein
• Relevance: Plays an essential role for activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA3A probably contributes to a negative autoregulatory control loop.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Activity: Not test
• Length: Partial
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 22.6 kDa
• References & Citations: "A structural basis for varied alphabeta TCR usage against an immunodominant EBV antigen restricted to a HLA-B8 molecule." Gras S., Wilmann P.G., Chen Z., Halim H., Liu Y.C., Kjer-Nielsen L., Purcell A.W., Burrows S.R., McCluskey J., Rossjohn J. J Immunol 188:311-321 (2012) .
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.