Recombinant Human Interferon-lambda1/Interleukin-29

• Background:

  IL-28A, IL-28B, and IL-29, also named interferon-λ2 (IFN-λ2), IFN-λ3, and IFN-λ1, respectively, are newly identified class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13 % a.a. sequence identity) and the type I IFN family (15-19 % a.a. sequence identity) . The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor β (IL-10 Rβ) and a novel IL-28 receptor α (IL-28 Rα, also known as IFN-λR1) . Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation.

• Product Name Alternative:

  Cytokine Zcyto21

• Gene ID:

  282618

• UniProt:

  Q8IU54

• Target:

  GPVPTSKPTT TGKGCHIGRF KSLSPQELAS FKKARDALEE SLKLKNWSCS SPVFPGNWDL RLLQVRERPV ALEAELALTL KVLEAAAGPA LEDVLDQPLH TLHHILSQLQ ACIQPQPTAG PRPRGRLHHW LHRLQEAPKK ESAGCLEASV TFNLFRLLTR DLKYVADGNL CLRTSTHPES T

• Purification:

  > 97 % by SDS-PAGE and HPLC analyses.

• Endotoxin:

  Less than 1 EU/ug of rHuIFN-λ1/IL-29 as determined by LAL method.

• Bioactivity:

  5

• Storage Conditions:

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

• Calculated Molecular Weight:

  Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.

• Formulation:

  Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

• Host or Source:

  Escherichia coli