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Anti-Miro2 Antibody

CAT: 1303-75-365Size: 100 µLDry Ice: NoHazardous: No
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CAT#:1303-75-365Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Mitochondrial Rho GTPase 2 (Miro2) or Ras Homolog Family Member T2 is encoded by the gene RHOT2 and is a member of the Rho family of GTPases. Miro2 is a mitochondrial GTPase. It is found on the outer mitochondrial membrane and is involved in mitochondrial trafficking. Miro2 is ubiquitously expressed in tissues. Diseases associated with this gene include 3-Methylglutaconic Aciduria and, Autosomal Recessive Congenital Hypomyelinating Neuropathy.
Description
Our Anti-Miro2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N384/63. It detects human and rat Miro2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Short Description
Our Anti-Miro2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N384/63. It detects human and rat Miro2, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.
Synonyms
Mitochondrial Rho GTPase 2 (MIRO-2) (hMiro-2) (EC 3.6.5.-) (Ras homolog gene family member T2)
Gene Name
RHOT2 ARHT2 C16orf39
UniProt
Q8IXI1
Host
Mouse
Reactivity
Human and Rat
Immunogen
Fusion protein amino acids 343-580 (second Miro domain) of human Miro2 (accession number Q8IXI1) produced recombinantly in E. Coli
Target
Miro2
Clonality
Monoclonal
Isotype
IgG1
Clone
N384/63
Type
Primary Antibody
Applications
WB, IHC, ICC, ELISA
Field of Research
Cell Signaling/G Protein Signaling/Mitochondrial Biology
Concentration
1 mg/mL
Format
Purified by Protein A chromatography
Buffer
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Molecular Weight
80 kDa
Shipping Conditions
Shipped on ice packs
Storage Conditions
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Notes
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Specificity
Does not cross-react with Miro1
Quality Control
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Human
WB Brain Quality Control
1
Conjugate
Unconjugated
RRID
AB_2315894

UniProtKB · Q8IXI1

Mitochondrial Rho GTPase 2

MIRO2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8IXI1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RHOT2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MIRO-2, hMiro-2
EC number
3.6.5.-
Processing
—
Secondary accessions
A2IDC2, Q8NF53, Q96C13, Q96S17, Q9BT60, Q9H7M8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumGTP-bindingMagnesiumMetal-bindingNucleotide-binding

Molecular function

Hydrolase

PTM

Isopeptide bondUbl conjugation

Cellular component

MembraneMitochondrionMitochondrion outer membrane

Domain

RepeatTransmembraneTransmembrane helix

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