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Anti-Doc2B Antibody

CAT: 1303-75-214Size: 100 µLDry Ice: NoHazardous: No
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CAT#:1303-75-214Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Double C2-like domain-containing protein beta or Doc2b is encoded by the gene DOC2b. Doc2b functions as a calcium sensor which positively regulates soluble NSF attachment receptor (SNARE) dependent fusion of vesicles with membranes. Doc2b binds phospholipids and may act at the priming stage of membrane fusion by modifying membrane curvature to stimulate fusion. Doc2b is expressed in pancreatic cells, endocrine cells and brain, where it is highly expressed in neurons. (Friedrich et al.Neurosci., July 2, 2008 • 28 (27) :6794 – 6806)
Description
Our Anti-Doc2b mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N150/21. It detects mouse and rat Doc2b, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.
Short Description
Our Anti-Doc2b mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N150/21. It detects mouse and rat Doc2b, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, ELISA.
Synonyms
Double C2-like domain-containing protein beta (Doc2-beta)
Gene Name
Doc2b
UniProt
P70610
Host
Mouse
Reactivity
Mouse and Rat
Immunogen
Fusion protein amino acids 1-216 (N-terminus) of rat Doc2b (accession number P70610) produced recombinantly in E. Coli
Target
Doc2b
Clonality
Monoclonal
Isotype
IgG1
Clone
N150/21
Type
Primary Antibody
Applications
WB, IHC, ICC, ELISA
Field of Research
Vesicle Trafficking/Fusion
Concentration
1 mg/mL
Format
Purified by Protein A chromatography
Buffer
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Molecular Weight
45 kDa
Shipping Conditions
Shipped on ice packs
Storage Conditions
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Notes
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
Specificity
Does not cross-react with Doc2a
Quality Control
Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Rat
Conjugate
Unconjugated
IHC Quality Control
1
RRID
AB_2315830

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