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Anti-Complement C3a/C3a (desArg) /C3 Antibody

CAT: 1303-GAU 013-16-010Size: 100 µLDry Ice: NoHazardous: No
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CAT#:1303-GAU 013-16-010Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg) .
Description
Our mouse Complement C3a/C3a (desArg) /C3 monoclonal antibody from Antibodies Incorporated is produced in-house from clone K13/16-5.7. It detects human Complement C3a/C3a (desArg) /C3 and is Protein A or Protein G purified. Works in ELISA, IF, IHC, WB.
Synonyms
C3, AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, acylation-stimulating protein cleavage product, complement component 3, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3
Gene Name
C3
NCBI Gene ID
718
UniProt
P01024
Host
Mouse
Reactivity
Human
Immunogen
Human C3a
Target
Complement C3a/C3a (desArg) /C3
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
K13/16-5.7
Type
Primary Antibodies
Applications
ELISA, IF, IHC, WB
Concentration
1 mg/mL ±15%
Format
Protein A or Protein G purified
Buffer
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Molecular Weight
187 kDA (Native C3), 9 kDa (C3a and C3a (desArg) )
Shipping Conditions
Shipped on ice packs
Storage Conditions
2-8°C without exposure to light
Notes
BSA free
IHC Dilution
0.14583333333333334
Specificity
GAU 013-16 is specific for human C3, C3a and C3a (desArg) (1) and does not cross-react with C4a or C5a. (2, 3) GAU 013-16 recognizes a different epitope on the 9 kDa C3a than GAU 017-01 (2, 3) . No reaction is seen with a synthetic octapeptide representing the C3a C-terminal. (2)
Brand
Antibodies Inc (via BioPorto acquisition)
Recommended Usage
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Physical Properties
Liquid
Immunogen Species
Human

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