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QuicKey Pro Human C5a (Complement Component 5a) ELISA Kit

CAT: 0763-E-OSEL-H0029-01Size: 24 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-OSEL-H0029-01Size:24 Tests
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Abbreviation
C5a
UniProt
P01031
Cross Reactivity
This kit recognizes C5a in samples.No significant cross-reactivity or interference between C5a and analogues was observed.
Target
C5a; Complement C5 alpha chain; CPAMD4; C5a anaphylatoxin
Type
Sandwich-ELISA
Applications
ELISA
Field of Research
Immunology
Assay Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human C5a are added to the micro ELISA plate wells. Human C5a in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human C5a.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C5a. The concentration of Human C5a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
1 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
46.88 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C,6 months
Detection Instrument
Microplate reader
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human C5a concentrations in serum, plasma and other biological fluids.

UniProtKB · P01031

Complement C5

CO5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01031
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q14CJ0, Q27I61
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Biological process

Complement alternate pathwayComplement pathwayCytolysisImmunityInflammatory responseInnate immunity

Disease

Disease variant

Cellular component

MembraneMembrane attack complexSecretedTarget cell membraneTarget membrane

Domain

Signal

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