Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit

• Target:

  F0F1-ATPase; ATP Synthase

• Type:

  Colorimetric method

• Field of Research:

  Mitochondria Function

• Assay Principle:

  Mitochondrial complex V is also known as F0F1-ATP synthase. ATP is hydrolyzed by F0F1-ATP synthase to produce ADP, and ADP converts NADH into oxidized coenzyme I (NAD) after enzyme conversion reaction. The activity of mitochondrial complex V can be quantified by measure the change OD value at 340 nm.

• Assay Performance Time:

  30 min

• Sample Type:

  Cell

• Detection Range:

  3.40-272.07 U/L

• Sensitivity:

  3.40 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  3.0-6.5%

• Intra CV:

  3.0-4.0%

• Detection Instrument:

  Microplate reader