Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Mitochondria Function; cuproptosis

• Assay Principle:

  Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The specific inhibitor rotenone can inhibit the enzyme, and the total enzyme activity minus the enzyme activity after inhibitor inhibition can reflect its specific enzyme activity.

• Assay Performance Time:

  15 min

• Sample Type:

  Animal tissue

• Detection Range:

  15.58-224.2 U/L

• Sensitivity:

  15.58 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  8.4%

• Intra CV:

  0.8%

• Detection Instrument:

  Microplate reader