Mitochondrial Complex IV (Cytochrome C Oxidase) Activity Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Mitochondria Function; cuproptosis

• Assay Principle:

  Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.

• Assay Performance Time:

  10 min

• Sample Type:

  Animal tissue

• Detection Range:

  3.0–88.6 U/L

• Sensitivity:

  3.0 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  9.5%

• Intra CV:

  5%

• Detection Instrument:

  Microplate reader