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Methylene Blue counter stain

CAT: 0013-GTR19315059Size: 100 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR19315059Size:100 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This methylene Blue counterstain solution is designed for nuclear counterstain after completion of Immunohistochemistry staining or for routine histology.
Storage Conditions
Room Temperature
Notes
For research use only.
Applications Notes
A. Immunohistochemistry: 1. After chromogen staining, wash slide 2-3X with de/di water, 2X with buffer and 2X with de/di. water 2. Apply Methylene Blue counterstain for 3-5 minutes or longer, if darker color is required. 3. Rinse with water, followed by alcohol gradients 30 to 95%; 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount (limonene) or equivalent. B. Histology: A. Follow the procedure as outlined for hitology 1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections. 2. At the counterstain step, apply Methylene Blue counterstain for 3-5 minutes or longer if darker color is required. 3. Rinse with water, dehydrate with gradients of alcool 30-95%; and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount or equivalent.
Tested Applications
IHC

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