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Yeast Poly (A) Polymerase

CAT: 0013-GTR19282629Size: 30000 UnitsDry Ice: NoHazardous: No
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CAT#:0013-GTR19282629Size:30000 Units
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Yeast Poly (A) Polymerase catalyzes the transfer of AMP to 3'-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+or Mn2+as cofactor. It works more efficiently than E.coli Poly (A) polymerase in some poly (A) tailing and RNA labeling reactions (e.g. shorter incubation time, broader acceptance of RNA template size) . Polyadenylation increases (m) RNA stability and therefore translation efficiency in transfection and microinjection experiments in eukary-otic cells. For information on (m) RNA polyadenylation using in vitro tran-scription reaction mixes as template, refer to the Poly (A) Tailing Enzyme Testkit (#orb611231) .
Product Name Alternative
Recombinant, E. coli overexpressing Saccharomyces cerevisiae Poly (A) Polymerase
Field of Research
Infectious Disease & Virology; Molecular Biology
Concentration
600 units/μl
Purity
≥ 95% (SDS-PAGE)
Form
Liquid
Storage Conditions
Store at -20 °C. avoid freeze/thaw cycles
Notes
For research use only.

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