Poly (A) RNA Polymerase

CAT: 1465-EG25108MSize: 500 UDry Ice: NoHazardous: No
CAT#:1465-EG25108MSize:500 U
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Poly (A) Polymerase is a polymerase derived from Escherichia coli, also known as E.coli PAP, PAP enzyme, Poly (A) tailing enzyme, or PolyA tailing enzyme. Poly (A) Polymerase catalyzes the rapid and efficient addition of a poly (A) tail to the 3' end of single-stranded RNA (ssRNA) in the presence of ATP. It can use various single-stranded RNA as substrate, while double-stranded RNA and excessively short oligonucleotides (<20 nt) are not recommended as substrates. DNA cannot be used as substrates for this enzyme. Poly (A) tailing reaction catalyzed by E. coli Poly (A) Polymerase can only use ATP, but not ADP or dATP. In addition, when CTP and UTP are used, the incorporation amount is less than 5% of that when using ATP. GTP can not be used by this enzyme.
Assay Protocol
http://en.best-enzymes.com/storage/upload/files/directions/16tiwaizhuanlu/EG25108.pdf
Storage Conditions
Store at -20°C for 2 years.

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