X-GAL

• Description:

  X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity[1][2].

• Product Name Alternative:

  BCIG

• UNSPSC:

  12171500

• Hazard Statement:

  H302, H315, H319, H335

• Target:

  Fluorescent Dye; Glycosidase

• Type:

  Dye Reagents

• Related Pathways:

  Metabolic Enzyme/Protease; Others

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/X-GAL.html

• Purity:

  99.88

• Solubility:

  DMF : 200 mg/mL (ultrasonic) |DMSO : 100 mg/mL (ultrasonic)

• Smiles:

  O[C@H]([C@@H](O)[C@H]1O)[C@@H](O[C@@H]1CO)OC2=CNC3=C2C(Cl)=C(Br)C=C3

• Molecular Formula:

  C14H15BrClNO6

• Molecular Weight:

  408.63

• Precautions:

  H302, H315, H319, H335

• References & Citations:

  [1]Sanchez-Ramos J, et al. The X-gal caution in neural transplantation studies. Cell Transplant. 2000 Sep-Oct;9 (5) :657-67.|[2]Li S, et al. In-situ SERS readout strategy to improve the reliability of beta-galactosidase activity assay based on X-gal staining in shortening incubation times. Talanta. 2021 Nov 1;234:122689.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  4°C (Powder, protect from light)

• Scientific Category:

  Dye Reagents

• Clinical Information:

  No Development Reported

• CAS Number:

  [7240-90-6]