Saikosaponin D

Saikosaponin is a novel SERCA inhibitor by inhibiting NF-κB and STAT3 signaling to protect against acetaminophen-induced hepatotoxicity. (In Vitro) :Saikosaponin D (Compound 3) is a triterpene saponin, which inhibits E-selectin, L-selectin and P-selectin binding to THP-1 cells, with IC50s of 1.8 μM, 3.0 μM and 4.3 μM, and such effects are not due to cytotoxic action. Saikosaponin D (1, 5, 10 μM) dose-dependently inhibits the THP-1 adhesion to the HUVECs monolayer activated by TNF-α. Saikosaponin D (30 μM) also inhibits the expression of P-selectin ligand (CD162) in THP-1 cells. Saikosaponin D (5 μM) suppresses the proliferation of HSC-T6 cells induced by H2O2 treatment, reduces the expression levels of α-SMA, TGF-β1, Hyp, COL1 and TIMP-1, and increases MMP-1 expressioon, thus inhibiting H2O2-induced excessive extracellular matrix (ECM) formation, with similar effects to estradiol (E2), and these effects are blocked by ER antagonists. Saikosaponin D also inhibits oxidative stress-induced ROS generation and down reduates MAPK signaling pathway, and the inhibition is also suppressed by ER antagonists. (In Vivo) :Saikosaponin D (2 mg/kg/day, i.p.) shows a protective effect on overdose of acetaminophen (APAP) -induced liver injury of mice. Saikosaponin D affects APAP metabolism, increases GSH levels but does not alter PPARα activation. Saikosaponin D (2 mg/kg/day, i.p.) also suppresses APAP-induced increases in the expression of STAT3 target genes and pro-inflammatory cytokines and inhibits APAP-induced activation of STAT3 and NF-kB.

20874-52-6

>98% (HPLC)

Pyridine, Methanol, Ethanol, etc.

C[C@@H]1[C@@H] ([C@@H] ([C@H] ([C@@H] (O1) O[C@H]2CC[C@]3 ([C@H] ([C@]2 (C) CO) CC[C@@]4 ([C@@H]3C=C[C@@]56[C@]4 (C[C@H] ([C@@]7 ([C@H]5CC (CC7) (C) C) CO6) O) C) C) C) O) O[C@H]8[C@@H] ([C@H] ([C@@H] ([C@H] (O8) CO) O) O) O) O

C42H68O13

780.98

Storage temperature: -20°C. Stability: ≥ 2 years