Coelenterazine

Coelenterazine is the luciferin, the light-emitting molecule, found in many aquatic organisms across seven phyla. It is the substrate of many luciferases such as Renilla reniformis luciferase (Rluc), Gaussia luciferase (Gluc), and photoproteins, including aequorin, and obelin. Coelenterazine was used: (1) For Aequorin assays; (2) For measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cells2; (3) . In Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity. Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca2+ binds to the complex; used to measure Ca2+ concentration in cells with high sensitivity and large dynamic range. (In Vitro) :HCT-8 control cells, transiently expressing Renilla luciferase (RLuc), showed low bioluminescence due to P-glycoprotein–mediated efflux transport of coelenterazine. By comparison, transiently expressing RLuc HCT-8 cells, wherein P-glycoprotein was down-regulated with shRNAi, showed high bioluminescence. (In Vivo) :The in vivo growth potential of HCC1806-RR was monitored by injecting animals with coelenterazine (2 mg/kg) i.v. and exposing them to a charged-coupled device (CCD) camera 5 minutes later. Rluc activity was detected as light emitted from the tumor cells and acquired as a pseudo-color image superimposed over a black and white photograph of the animal. All mice demonstrated very high Rluc activity at the primary site with the majority of mice simultaneously showing metastases to inguinal ILNs.

55779-48-1

Oplophorus, luciferin | Coelenterazine | CLZN

>98% (HPLC)

In Vitro: Ethanol : 1 mg/mL (2.36 mM)

Oc1ccc (Cc2nc3c (Cc4ccccc4) [nH]c (cn3c2=O) c2ccc (O) cc2) cc1

C26H21N3O3

423.47

Storage temperature: -20°C. Stability: ≥ 2 years

For research use only.