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AMCA-6-dUTP

CAT: 0013-GTR19152163Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR19152163Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AMCA-6-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended AMCA-6-dUTP/dTTP ratio for PCR and Nick Translation: 20-30% AMCA-6-dUTP/80-70% dTTP (PCR), 30-50% AMCA-6-dUTP/70-50% dTTP (Nick Translation) .
Product Name Alternative
Aminoallyl-dUTP-AMCA, Aminomethylcoumarin-6-dUTP, Aminomethylcoumarin-5-aminoallyl-2-deoxy-uridine-5-triphosphate, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; pH: 7.5 ± 0.5
Smiles
C (/C=C/c1cn (c (=O) [nH]c1=O) [C@H]1C[C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O) NC (=O) CCCCCNC (=O) Cc1c (c2c (oc1=O) cc (cc2) N) C
Molecular Formula
C30H40N5O18P3
Molecular Weight
Theoretical MW: 851.58 g/mol (free acid) ; Detected MW: 851.16 g/mol (free acid)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 350 nm, λem 450 nm, ε 19.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .

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T75382-01AMCA-6-dUTP
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