SARS-CoV-2 Spike S1 Protein, His Tag (BA.4 & BA.5/Omicron)

SARS-CoV-2 Spike S1 Protein, His Tag (BA.4 & BA.5/Omicron)

• Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Description: SARS-CoV-2 Spike S1, His Tag (BA.4 & BA.5/Omicron) (S1N-C52Hy) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.4 and BA.5).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.5 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Val 16 - Arg 685
• Host: HEK 293
• Target: Spike S1
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: SARS-CoV-2
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized SARS-CoV-2 Spike S1, His Tag (BA.4 & BA.5/Omicron) (Cat. No. S1N-C52Hy) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-4 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 76.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C