β-1,3-Glucanase Activity Colorimetric Assay Kit

β-1,3-Glucanase Activity Colorimetric Assay Kit

• Field of Research: Plant Physiology Series
• Detection Method: Colorimetric method
• Assay Principle: β-1, 3-glucanase (β-1,3-GA, EC 3.2.1.39) is mainly found in plants and catalyzes the hydrolysis of β-1, 3-glucosidic bonds, thereby destroying fungal cell walls. In particular, when synergistic with chitinase, it can significantly inhibit the growth of fungi. When plants are infected with diseases or under other stress conditions, they can induce a large amount of β-1, 3-GA to enhance plant resistance to adverse external stimuli. Therefore, the determination of β-1, 3-GA activity is widely used in plant pathology and stress physiology research.
  β-1,3-GA hydrolyses the β-1, 3-glucosidic linkage of laminarin to produce reducing ends. The activity can be reflected by measuring the amount of reducing sugar using 3, 5-dinitrosalicylic acid.
• Assay Performance Time: 50 min
• Sample Type: Plant tissue
• Detection Range: 6.22-333.33 U/mL
• Shipping Conditions: Ice bag
• Storage Conditions: This product can be stored at 2-8°C for 12 months with shading light.
• Applications Notes: Enzyme activity