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Recombinant Human IL-2 Protein

CAT: 0013-GTR19125986-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19125986-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding Pro22-Thr153 (Cys145Ser) is expressed.
Product Name Alternative
Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin
UniProt
P60568
Expression Region
Pro22-Thr153 (Cys145Ser)
Tag
Tag free
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Human IL-2RA-His (Cat#orb2994464) on HIS1K Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 31 nM as determined in BLI assay. Loaded Human IL-2RA-Fc (Cat#orb2994463) on Protein A Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 13.2 nM as determined in BLI assay. Loaded Human IL-2RB-His (Cat#orb2994462) on HIS1K Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 0.19 uM as determined in BLI assay. Loaded Human IL-2RB-Fc (Cat#orb2994461) on Protein A Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 0.27 uM as determined in BLI assay. Loaded Mouse IL-2RA-His (Cat#orb2994413) on HIS1K Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 33 nM as determined in BLI assay. Loaded Biotinylated Human IL-2RB-His-Avi (Cat#orb2993868) on HIS1K Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 0.34 uM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using CTLL‐2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg/ml.
Form
Lyophilized
Molecular Weight
Predicted: 15.5 KDa. Observed: 14 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 10mM Acetata-Na, 5% Trehaiose, pH 4.5.

UniProtKB · P60568

Interleukin-2

IL2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P60568
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-2
EC number
—
Processing
Precursor
Secondary accessions
P01585
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Chromosomal rearrangement

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Disease

Proto-oncogene

Cellular component

Secreted

Domain

Signal

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