Mouse anti Rat SNAP25

• Background:

  SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells. These two isoforms differ by 9 amino acids in the central portion of the protein.

• Synonyms:

  Synaptosome-Associated Protein of 25 kDa

• UniProt:

  P60880

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat, Monkey, Chicken, Cow

• Isotype:

  IgG1

• Clone:

  S-7B8

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Source:

  Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with rat brain synaptosomal membranes and mouse NS1 cells.

• Applications:

  WB, IHC (paraffin), ICC

• Field of Research:

  Signal Transduction

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  Antibody can be used for Western blotting (1-5 µg/mL), immunocytochemistry and immunohistochemistry on paraformaldehyde fixed CNS tissues (1-5 µg/mL). Does not stain reduced proteins. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Ritchie, T.C., et al. 'A nerve terminal protein with a selective distribution in spinal cord and brain.' J. Neurosci. 1989: 9, 2697-2709_x000D_ 2. Zhao, N., et al. 'Cloning and sequence analysis of the Human SNAP25 cDNA.' Gene 1994: 145, 313-314 _x000D_ 3. McMahon , H.T., et al. 'Synaptic core complex of synaptobrevin, syntaxin and SNAP25 forms high affinity alpha-SNAP binding site.' J. Biol. Chem. 1995: 270, 2213-2217_x000D_ 4. Gerona, R.R., et al. 'The C terminus of SNAP25 is essential for Ca(2+)-dependent binding of synaptotagmin to SNARE complexes.' J. Biol. Chem. 2000: 275, 6328-6336

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4