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Recombinant Human ROR1 Protein

CAT: 0013-GTR19124671-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19124671-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human Inactive Tyrosine-protein Kinase Transmembrane Receptor ROR1 is produced by our Mammalian expression system and the target gene encoding Gln30-Glu403 is expressed with a 6His tag at the C-terminus.
Product Name Alternative
Inactive tyrosine-protein kinase transmembrane receptor ROR1; Neurotrophic tyrosine kinase, receptor-related 1; ROR1; NTRKR1
UniProt
Q01973
Expression Region
Gln30-Glu403
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Stem Cell & Developmental Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human ROR1 (C-6His) (Cat#orb2994090) at 2 μg/ml (100 μl/well) can bind Human ROR1 Antibody (3E8) (Cat#orb2992600) . The ED50 of Human ROR1 Antibody (3E8) (Cat#orb2992600) is 1.69 ng/ml. (Regularly tested) . BLI: Loaded Human ROR1 Antibody (3E8) -Fc (Cat#orb2992600) on Protein A Biosensor, can bind Human ROR1-His (Cat#orb2994090) with an affinity constant of 6.35 nM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human ROR1 (C-6His) (Cat#orb2994090) on HIS1K Biosensor, can bind Human ROR1 Antibody (3E8) (Cat#orb2992600) with an affinity constant of 0.163 pM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 42.8 KDa. Observed: 60-80 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Citrate, 5% Trehalose, 1mM EDTA, 0.02% Tween80, pH 5.5.

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