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Recombinant Human GPC3 Protein

CAT: 0013-GTR19125495-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19125495-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human Glypican-3 is produced by our Mammalian expression system and the target gene encoding Gln25-His559 is expressed with a 6His tag at the C-terminus.
Product Name Alternative
Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; GPC3; OCI5
UniProt
P51654
Expression Region
Gln25-His559
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Bioactivity
ELISA: Immobilized Human FGFb (146AA) (Cat. No.:orb3002475) at 2 μg/ml (100 μl/well) can bind Human GPC3 (C-6His) (Cat. No.:orb2995093) . The EC50 of Human GPC3 (C-6His) (Cat. No.:orb2995093) is not higher than 50 ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 61.6 KDa. Observed: 70-150&38 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS,4%Mannitol, pH 7.4.

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