Plasma Sample Diluent (Antigen-Down SD)

• Description:

  By accurately reading and quantitating high-titer samples. High-titer samples may overload the finite binding capacity of the coated ELISA plate surface, thus requiring dilution prior to testing in the assay.

• Label:

  ICT

• Type:

  ELISA & Assay Reagents

• Assay Protocol:

  Simply dilute the buffer 1:10 (100mL WB to 900mL diH20) and it’s ready to use. To rinse microtiter plates, the buffer may be dispensed through a squirt bottle, a plate washer, or a multi-channel pipette. Be sure to fill the Wells all the way up (400uL for a 96-Well plate). We do NOT recommend submerging the entire plates in a bath as many of the Wells become contaminated, which decreases the reliability of the assay.

• Concentration:

  1X

• Buffer:

  7.4 at 1X

• pH:

  7.4 at 1X

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  2-8°C

• Shelf Life:

  Expires two years from date of manufacture

• Target Description:

  Plasma Sample Diluent is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This diluent contains proprietary additives in a buffered BSA protein base with which provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. Additional additives have been included to inhibit thrombin (clotting) and complement activity during the plasma sample incubation period. ESD200 also contains an antimicrobial agent to provide a solution free of bacterial contamination.