Protein-Free Sample Diluent

CAT: 0436-6704Size: 1 LDry Ice: NoHazardous: No
CAT#:0436-6704Size:1 L
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Description
Protein-Free Sample Diluent is a protein-free matrix used for the dilution of biological samples (e.g. serum, cell culture media) into the useful range of antibody-sandwich or antigen-down ELISA format assays. This unique buffer contains a heterogeneous mixture of proprietary molecules that help reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate Well surface.
Label
ICT
Type
ELISA & Assay Reagents
Assay Protocol
1. Protect the HRP Conjugate Stabilizer from light. This may be done by covering the container with aluminum foil., 2. Add the HRP-conjugated protein at the target concentration to HRP Conjugate Stabilizer. For example, if diluting an HRP-conjugated antibody 1:5000, add 10 µL conjugate stock to 49.99 mL HRP Conjugate Stabilizer., 3. Mix for 15 minutes., 4. Use the diluted HRP conjugate in the immunoassay., 5. Store remaining HRP conjugate solution at 2-8°C. Protect from direct exposure to light.
Concentration
1X ready to use
pH
pH 7.1-7.6
Additionnal Information
ELISA & Assay Reagents ; Sample and Assay Diluents
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Shelf Life
Expires two years from date of manufacture
Target Description
Sample diluents are used to dilute samples into the functional range of the assay and to create the standard curve. Due to the finite binding capacity of the plate Well-coated proteins (e.g., antibodies, antigens), highly concentrated samples must be diluted in order to obtain absorbance readings within the sensitivity detection limits of the instrument. Properly formulated sample diluents will also reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate Well surface., , Protein-Free Sample Diluent provides a non-protein-based buffered, neutral pH environment that is highly compatible with antibody-antigen interactions. Antimicrobial agents allow for room temperature bench-top use and extensive storage stability at 2-8°C.

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