Peroxidase, Unconjugated - Bader says not IHC Reagent?

• Description:

  Horseradish peroxidase (HRP) is a glycoprotein, MW approximately 44 kDa, with hemin prosthetic group and bound Ca++. Peroxidase oxidizes many chromogenic substrates (ABTS, TMB, o-dianiside, 5-Aminosalicylic acid (5AS), DAB) to form color complexes; chemiluminescent (luminal and isoluminol) and fluorogenic (tyramine, homovanillic acid and 4-hydroxyphenyl acetic acid). These are used in ELISA, immunoblots, and Immunohistochemistry.

• Other Statements:

  For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

• Label:

  ICT

• Type:

  IHC Reagents

• Applications:

  IHC

• Assay Protocol:

  The product is supplied as salt free in distilled water. No preservative have been added. On arrival aliquot and freeze at -20oC. Proclin 300 (final concentration 0.05%) can be added as a preservative and then the enzyme can be stored at 2-8oC. DO NOT USE Sodium azide as preservative in buffers or washing steps as it will inhibit the enzyme activity.

• Buffer:

  6.0-6.5

• pH:

  6.0-6.5

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  -20°C

• Target Description:

  Horseradish peroxidase (HRP) is a glycoprotein, MW approximately 44 kDa, with hemin prosthetic group and bound Ca++. Peroxidase oxidizes many chromogenic substrates (ABTS, TMB, o-dianiside, 5-Aminosalicylic acid (5AS), DAB) to form color complexes; chemiluminescent (luminal and isoluminol) and fluorogenic (tyramine, homovanillic acid and 4-hydroxyphenyl acetic acid). These are used in ELISA, immunoblots, and Immunohistochemistry.