Plasma Sample Diluent (Antigen-Down SD)

CAT: 0436-ESD200-2Size: 500 mLDry Ice: NoHazardous: No
CAT#:0436-ESD200-2Size:500 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
By accurately reading and quantitating high-titer samples. High-titer samples may overload the finite binding capacity of the coated ELISA plate surface, thus requiring dilution prior to testing in the assay.
Label
ICT
Type
ELISA & Assay Reagents
Assay Protocol
Simply multiply the value determined from the curve by your dilution factor. For example, if you diluted your sample 1:2 (250uL sample into 250uL sample diluent) and this yielded an OD of 0.437, when read off the curve, the OD of 0.437 correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution factor) yielding a value of 400pg/mL, meaning that the original starting sample had 400pg/mL of analyte present.
Concentration
1X
Buffer
7.4 at 1X
pH
7.4 at 1X
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Shelf Life
Expires two years from date of manufacture
Target Description
Plasma Sample Diluent is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This diluent contains proprietary additives in a buffered BSA protein base with which provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. Additional additives have been included to inhibit thrombin (clotting) and complement activity during the plasma sample incubation period. ESD200 also contains an antimicrobial agent to provide a solution free of bacterial contamination.

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