Cathepsin B

• Description:

  Cathepsin B purified from human liver tissue is best used to study enzyme kinetics, cleave target substrates, and screen for inhibitors.

• Label:

  ICT

• Cell Type:

  Figure 1. Optimization of double-antigen sandwich ELISA. (a) Capture (0.5 to 5.0 µg/mL) and detection antigen (0.2 (green), 0.4 (light blue), 0.8 (yellow), and 1.6 (purple)) were used to optimize capture and detection antigen (N protein). Capture antigen was diluted with Antigen Coating Buffer. From Figure 1a of Cordero-Ortiz, M, et al. (2023) Animals (Basel) 13, 3487 doi: 10.3390/ani13223487. Used under CC License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2023 by the authors.

• Type:

  Cell Viabiity

• Concentration:

  25 μg protein in 45.5 μl 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  -80°C

• Notes:

  Ships on Dry Ice; 13% discount

• Target Description:

  Cathepsin B, a member of the papain family of cysteine proteases, has also been called Cathepsin B1 and APP secretase. It is a ubiquitous lysosomal protease that functions in protein degradation and apoptosis. It possesses both endopeptidase and dipeptidyl carboxypeptidase activites. It can also be secreted from the cell and is implicated in disease states such as arthritis, gingivitis, and tumor metastasis. Cathepsin B is best used to study enzyme kinetics, cleave target substrates, and screen for inhibitors.