Recombinant Caspase 3 Monoclonal Antibody

• Background:

  Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

• Description:

  This is a Recombinant Caspase 3 Recombinant Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  A830040C14Rik, Apopain, CASP-3, CASP3, CASP3, Casp3a, Caspase 3, Caspase 3, apoptosis-related cysteine peptidase, Caspase 3, apoptosis-related cysteine protease, Caspase 3, apoptosis-related cysteine protease a, Caspase-3 subunit p12, CC3, CPP-32, CPP32, CPP32B, Cysteine p

• Gene ID:

  836

• Swiss Prot:

  P42574

• Reactivity:

  Human, Mouse

• Immunogen:

  A synthetic peptide of human Caspase 3

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Type:

  Recombinant Antibody

• Applications:

  WB, IHC-P

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  300 μg/mL

• Dilution:

  WB 1:500-1:1000, IHC 1:50-1:100

• Buffer:

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  86kDa

• Observed Molecular Weight:

  86kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG