Anti-KLH | Keyhole Limpet Hemocyanin (monoclonal, clone 6D12)

Anti-KLH | Keyhole Limpet Hemocyanin (monoclonal, clone 6D12)

• Background: Keyhole limpet hemocyanin(KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea molluskMegathura crenulata. This extracellular respiratory protein  has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells,  monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Reacts with Keyhole Limpet 8-9 kDa Hemocyanin protein.
• Immunogen: Hemocyanin fromMegathura crenulata.
• Clonality: Monoclonal
• Applications: ELISA (ELISA), Western blot (WB)
• Dilution: 1:10 000 - 1:100 000 (ELISA), 1: 500 - 1: 5000 (WB)
• Purity: Purified on Protein A to a total immunoglobulin fraction, in Tris 0.1 M, glycine 0.1 M and 2% sucrose.
• Format: Lyophilized
• Reconstitution: Reconstitute in distilled water.
• Molecular Weight: ca. 400 kDa/subunit
• Precautions: Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglundet al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.
• Storage Conditions: Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.