Anti-mNeonGreen | Fluorescent Protein

• Background:

  MNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP. It has an excitation maximum at 506 nm and an emission maximum at 517 nm and therefore is compatible with the most GFP filter sets. mNeonGreen is 3x brighter compare to GFP, more stable and does not bleach so fast as GFP, which makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has MW of 26.6 kDa.

• HS Code:

  38221900

• UniProt:

  A0A1S4NYF2

• Host:

  Rabbit

• Reactivity:

  MNG tag in plant (Arabidopsis thaliana) and algal vectors (Chlamydomonas reinhardtii)

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known.

• Immunogen:

  KLH-conjugated peptide derived from synthetic peptide, UniProt: A0A1S4NYF2 from common lancelet Branchiostoma lanceolatum

• Clonality:

  Polyclonal

• Applications:

  Immunofluorescence (IF), Western blot (WB)

• Field of Research:

  BFP/FITC/GFP/RFP/TRITC/YFP

• Dilution:

  1 µg/1ml (IF), 1 : 1000 - 1: 5000 (WB)

• Purity:

  Immunogen affinity purified serum, in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl, of sterile water.

• Molecular Weight:

  Depends upon used fusion partner

• Precautions:

  This antibody is detecting protein sequence of mNG tag in plant and algal vectors.This antibody is also reacting to some degree with YFP and mCherry.

• Additionnal Information:

  The peptide used to elicit this antibody is conserved in the following expression vectors: dCas9-P2A-DHFR (TSc3) -T2A-mNeonGreen [Cloning vector pBM020]Cas9-P2A-DHFR (TSc3) -T2A-mNeonGreen [Cloning vector pBM006]mNeonGreen4-tDeg [Cloning vector pminiCMV-mNeonGreen4-tDeg]ER-localized mNEONGREEN [Binary vector pKT-NM-erNEON]mNeonGreen-3xFLAG [Cloning vector pLM160-mNeonGreen]mNeonGreen-ty1 [Cloning vector pSAG1-mNeonGreen_TUB1-dTomato]mNeonGreen, partial [Binary vector pRATIO2131]The peptide used to elicit this antibody is not conserved in GFP protein sequence. Antibody is also recognzing mCheery sequence.

• References & Citations:

  To be added when available, antibody available in November 2021.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• Prediction Reactivity:

  Species of your interest not listed? Contact us

• CAS Number:

  9007-83-4