Anti-mNeonGreen | Fluorescent Protein

Anti-mNeonGreen | Fluorescent Protein

• Background: mNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP.  It has an excitation maximum at 506 nm and an emission maximum at 517 nm and therefore is compatible with the most GFP filter sets.mNeonGreen is 3x brighter compare to GFP, more stable and does not bleach so fast as GFP, which makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has MW of 26.6 kDa.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: mNG tag in plant (Arabidopsis thaliana) and algal vectors (Chlamydomonas reinhardtii)
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known.
• Immunogen: KLH-conjugated peptide derived from synthetic peptide, UniProt:A0A1S4NYF2from common lanceletBranchiostoma lanceolatum
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Western blot (WB)
• Dilution: 1 µg/1ml (IF), 1 : 1000 - 1: 5000 (WB)
• Purity: Immunogen affinity purified serum, in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl, of sterile water.
• Molecular Weight: Depends upon used fusion partner
• Precautions: This antibody is detecting protein sequence of mNG tag in plant and algal vectors.This antibody is also reacting to some degree with YFP and mCherry.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.