Anti-RFP | Red Fluorescent Protein (clone RF5R)

Anti-RFP | Red Fluorescent Protein (clone RF5R)

• Background: RFP(Red Fluorescent Protein)is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated RFP from theDiscosoma sp.(sea anemone) N-terminal peptide
• Clonality: Monoclonal
• Applications: Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1 : 1000-3000 (WB)
• Purity: Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.
• Format: Liquid
• Precautions: Antibody is purified on Protein A.Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.
• References & Citations: Cecchiniet al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.
• Storage Conditions: Store at -20°C for up to 2 years.